Throughout the play many traumatic events have taken place, stating with Duncan’s murder and all the deaths following caused my Macbeth, all this guilt has fallen heavily onto lady Macbeth’s shoulders provoking her to commit suicide. At the beginning of the play, Lady Macbeth was very power-hungry, calling to evil spirits to “unsex me here, and fill me, from crown to the toe, top-full of direst cruelty!” to make her a man to she could kill Duncan herself. Over the course of the play she slowly deteriorates, a doctor is called to the castle after lady Macbeth repeatedly tries to wash her hands in her sleep “out, damned spot! out, I say! – one; two; why, then ‘t is time to do ‘t … here’s the smell of the blood still: all the perfumes of Arabia could not sweeten this little hand.” by the use of commas you can tell the urgency and frustration Lady Macbeth is feeling, opposed to when she was assertive and bold at the start.